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. 2019 Oct 1;10(55):5727–5729. doi: 10.18632/oncotarget.27230

Figure 7. Effects of aloe-emodin on anti-tumor activity.

Figure 7

(A) SkBr3 cells were used to establish xenografts in male BALB/c nude mice. Animals (six mice/group) were given control and AE (12.5, 25, and 50 mg/kg) by i.p. injection 5 times for 14–18 days. Tumor size was monitored through serial caliper measurements twice a week. Each point represents mean tumor size ± SE. (B) One representative mouse and its tumors are shown. (C) Representative tumors in each group are demonstrated. (D) Tumor weight was calculated as indicated in Materials and methods section. (E) Weekly body weight measurements indicated that therapy was not toxic. Each point represents mean ± SE. (F) Tumor tissues were immunoblotted with anti-HER-2, anti-YB-1, and anti-E-cadherin antibodies. (G) Tumor tissue was collected at the conclusion of therapy, fixed in 10% normal buffered formalin, and embedded in paraffin. Four-micron (4 μM) sections of tumor tissue were assessed using immunohistochemistry for androgen receptor expression. Immunohistochemical analyses in xenograft tumors on day 28 after AE treatment were performed using antibodies against HER-2, YB-1, and Ki67. Magnification, ×40; scale bar, 500 μM.