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. Author manuscript; available in PMC: 2019 Oct 7.
Published in final edited form as: Methods Mol Biol. 2016;1496:63–74. doi: 10.1007/978-1-4939-6463-5_6

Figure 1.

Figure 1.

Schematic of the two-stage incubation system to reconstitute COPI vesicles and tubules. In the first stage, Golgi membrane is incubated with purified ARF1 and coatomer. In the second stage, the incubated Golgi membrane is further incubated with purified ARFGAP1 and BARS to form COPI vesicles. For COPI tubule formation, the second-stage incubation involves the further addition of either purified cPLA2α or Cdc42.