KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| Escherichia coli OP50 | Caenorhabditis Genetics Center (CGC) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| CaCl2 | Fluka Analytical | Cat#21114 |
| MgCl2 | Fluka Analytical | Cat#63020 |
| NaCl | Sigma-Aldrich | Cat#S9888 |
| NaHCO3 | Sigma-Aldrich | Cat#S6014 |
| Sucrose | Sigma | Cat#S9378 |
| KCl | Sigma | Cat#P5405 |
| NaH2PO4 | Sigma-Aldrich | Cat#S0751 |
| Glucose | Sigma | Cat#G8270 |
| CsCl | Sigma | Cat#C3309 |
| CsF | Aldrich | Cat#289345 |
| EGTA | Sigma | Cat#E3889 |
| HEPES | Sigma | Cat#H4034 |
| Na2ATP | Sigma-Aldrich | Cat#2383 |
| CsOH | Aldrich | Cat#C8518 |
| Acetylcholine chloride | Sigma | Cat#A6625 |
| γ-Aminobutyric acid | Sigma | Cat#A2129 |
| Agarose | Sigma | Lot #SLBR6299V |
| All trans-Retinal | Sigma-Aldrich | CAS #116-31-4 |
| Experimental Models: Organisms/Strains | ||
| C. elegans: N2 | Caenorhabditis Genetics Center | N2 |
| C. elegans: unc-13(s69) I | Caenorhabditis Genetics Center | BC168 |
| C. elegans: unc-64(e246) III | Caenorhabditis Genetics Center | CB246 |
| C. elegans: tom-1 (nu468) I | Dybbs et al., 2005 | KP3293 |
| C. elegans: tom-1 (nu468) I; unc-64(e246) III | This paper | ZTH585 |
| C. elegans: cpx-1(ok1552) I | Caenorhabditis Genetics Center | RB1367 |
| C. elegans: nuEx1515 [Psnb-1::UNC-13L];unc-13(s69) I | Hu et al., 2013 | KP6893 |
| C. elegans: cpx-1(ok1552) I; unc-64(e246) III | This paper | ZTH390 |
| C. elegans: hztEx41 [Psnb-1::UNC-13L(ΔX)]; unc-13(s69) I | This paper | ZTH318 |
| C. elegans: tauEx441 [Psnb-1::UNC-13L(ΔC1)]; unc-13(s69) I | Michelassi et al., 2017 | JSD1067 |
| C. elegans: tauSi3 [Psnb-1::UNC-13L(ΔC2B)]; unc-13(s69) I | Michelassi et al., 2017 | JSD0805 |
| C. elegans: hztEx19 [Psnb-1::UNC-13L(H696K)]; unc-13(s69) I | This paper | ZTH9 |
| C. elegans: tauEx313 [Psnb-1::UNC-13L(D3,4N)]; unc-13(s69) I | Michelassi et al., 2017 | JSD0835 |
| C. elegans: tauEx321 [Psnb-1::UNC-13L(D3,4E)]; unc-13(s69) I | Michelassi et al., 2017 | JSD0849 |
| C. elegans: hztEx50 [Psnb-1::UNC-13L(D1-5N)]; unc-13(s69) I | This paper | ZTH555 |
| C. elegans: hztEx51 [Psnb-1::UNC-13L(ΔCaM)]; unc-13(s69) I | This paper | ZTH497 |
| C. elegans: hztEx52 [Psnb-1::UNC-13L(W593R)]; unc-13(s69) I | This paper | ZTH446 |
| C. elegans: hztEx57 [Psnb-1::sUNC-13]; unc-13(s69) I | This paper | ZTH484 |
| C. elegans: hztEx58 [Psnb-1::sUNC-13(Δlinker)]; unc-13(s69) I | This paper | ZTH480 |
| C. elegans: hztEx109 [Psnb-1::sUNC-13(ΔC2A)]; unc-13(s69) I | This paper | ZTH696 |
| C. elegans: hztEx92 [Psnb-1::sUNC-13(Δlinker1)]; unc-13(s69) I | This paper | ZTH523 |
| C. elegans: hztEx93 [Psnb-1::sUNC-13(Δlinker2)]; unc-13(s69) I | This paper | ZTH519 |
| C. elegans: hztEx94 [Psnb-1::sUNC-13(Δlinker3)J; unc-13(s69) | This paper | ZTH536 |
| C. elegans: hztEx59 [Punc-129::UNC-13L::mCherry]; nuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH108 |
| C. elegans: hztEx60 [Punc-129::sUNC-13::mApple]; nuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH584 |
| C. elegans: hztEx61 [Punc-129::sUNC-13(Δlinker)::mApple]; nuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH583 |
| C. elegans: hztEx66 [Punc-129::sUNC-13(ΔC2A)::mApple]; nuIs165 [Punc-129::UNC-10::GFP] | This paper | ZTH702 |
| C. elegans: nuEx1515 [Psnb-1::UNC-13L]; unc-13(s69) I; oxSi91 [Punc-17::ChIEF::mCherry] | This paper | ZTH504 |
| C. elegans: hztEx57 [Psnb-1::sUNC-13]; unc-13(s69) I; oxSi91 [Punc-17::ChIEF::mCherry] | This paper | ZTH525 |
| C. elegans: hztEx58 [Psnb-1::sUNC-13(Δlinker)]; unc-13(s69) I; oxSi91 [Punc-17::ChIEF::mCherry] | This paper | ZTH506 |
| C. elegans: hztEx62 [Psnb-1::UNC-64]; unc-64 (e246) III | This paper | ZTH418 |
| C. elegans: hztEx63 [Psnb-1::UNC-64(open)::SL2::mApple]; unc-64 (e246) III | This paper | ZTH513 |
| C. elegans: hztEx57 [Psnb-1::sUNC-13];unc-13(s69) I; unc-64(e246) III | This paper | ZTH511 |
| C. elegans: hztEx57 [Psnb-1::sUNC-13]; unc-13(s69) I; hztEx63 [Psnb-1::UNC-64(open)]; unc-64(e246) III | This paper | ZTH556 |
| C. elegans: hztEx63 [Psnb-1::UNC-64(open)::SL2::mApple]; unc-13(s69) I; unc-64(e246) III | This paper | ZTH684 |
| C. elegans: hztEx67 [Psnb-1::UNC-64::SL2::mApple]; unc-13(s69) I; unc-64(e246) III | This paper | ZTH703 |
| Oligonucleotides | ||
| UNC-13L(C2A) forward primer for fusion: acatggtaccatggat gacgttggagattacaatgatg | IDT | N/A |
| UNC-13L(C2A) reverse primer for fusion: gcacagtacttacatc aaaaggaagctcaaatcgaac | IDT | N/A |
| UNC-13L(R) forward primer for fusion: ttttgatgtaagtactgtgc ttgacgggaacg | IDT | N/A |
| UNC-13L(R) reverse primer for fusion: cggcggccgcctatgttc gattgatgttttgacttgatgc | IDT | N/A |
| Recombinant DNA | ||
| Psnb-1::UNC-13L | This paper | Phzt10 |
| Psnb-1::UNC-13L (ΔX) | This paper | Phzt41 |
| Psnb-1::UNC-13L(ΔC1) | Michelassi et al., 2017 | JP975 |
| Psnb-1::UNC-13L(ΔC2B) | Michelassi et al., 2017 | JP679 |
| Psnb-1::UNC-13L(H696K) | This paper | Phzt19 |
| Psnb-1::UNC-13L(D3,4N) | Michelassi et al., 2017 | JP772 |
| Psnb-1::UNC-13L(D3,4E)] | Michelassi et al., 2017 | JP786 |
| Psnb-1::UNC-13L(D1x-5N) | This paper | Phzt417 |
| Psnb-1::UNC-13L(ΔCaM) | This paper | Phzt381 |
| Psnb-1::UNC-13L(W593R) | This paper | Phzt355 |
| Psnb-1::sUNC-13 | This paper | Phzt448 |
| Psnb-1::sUNC-13(Δlinker) | This paper | Phzt420 |
| Psnb-1::sUNC-13(ΔC2A) | This paper | Phzt501 |
| Psnb-1::sUNC-13(Δlinker1) | This paper | Phzt526 |
| Psnb-1::sUNC-13(Δlinker2) | This paper | Phzt503 |
| Psnb-1::sUNC-13(Δlinker3) | This paper | Phzt575 |
| Punc-129::UNC-13L::mCherry | This paper | A011 |
| Punc-129::sUNC-13::mApple | This paper | Phzt653 |
| Punc-129::sUNC-13(Δlinker)::mApple | This paper | Phzt648 |
| Punc-129::sUNC-13(ΔC2A)::mApple | This paper | Phzt1018 |
| Psnb-1::UNC-64 | This paper | Phzt238 |
| Psnb-1::UNC-64(open)::SL2::mApple | This paper | Phzt458 |
| Psnb-1::UNC-64::SL2::mApple | This paper | Phzt459 |
| Software and Algorithms | ||
| ImageJ | Schneider et al., 2012 | N/A |
| PatchMaster | HEKA Elektronik | V2×73.2 |
| Igor Pro | Wavemetrics | Version 7 |
| SigmaPlot | Systat Software Inc. | Version 13.0 |
| Other | ||
| Microscope Cover Glass | Fisher Scientific | Lot#060214-9 |
| Glass Capillaries | World Precision Instruments, Inc. | Lot#2009330 |