Figure 2.

IgG isolated from renal immunodeposits of patients with IgAN but not from renal immunodeposits of patients with MN is specific for Gd-IgA1. Individual IgG samples from two patients with MN, pooled samples for four patients with IgG+RIF IgAN (pool 3), and five patients with IgG-RIF IgAN (pool 7) were assessed in two tests using Gd-IgA1 as the antigen: IgG-IgA1 immune-complex assay and IgG autoantibody assay. One unit (U) was defined as the amount of IgG resulting in optical density (OD) at 490 nm equal to 1.0. Results of the two assays for washes and extracts show excellent correlation (r²=0.98) by Pearson test. Filled circles denote extracts from the two pools of IgAN biopsies; filled squares denote extracts from two MN biopsies. Empty circles denote washes from the same two pools of IgAN biopsies, and empty squares denote washes from the same two MN biopsies.