Table 3.
IgG isolated using the one-wash protocol from renal immunodeposits of pooled remnant frozen kidney-biopsy specimens from patients with IgAN but not from immunodeposits of individual remnant frozen kidney-biopsy specimens of two patients with MN is specific for Gd-IgA1
| Sample | Immune-Complex Assay (U/µg IgG) | IgG Autoantibody Assay (U/µg IgG) |
|---|---|---|
| MN 1 W | 2 | 0.26 |
| MN 2 W | 9 | 0.07 |
| MN 1 Ext | 15 | 5.32 |
| MN 2 Ext | 26 | 2.34 |
| IgAN IgG+RIF W (pool 3) | 11 | 0.17 |
| IgAN IgG−RIF W (pool 7) | 1 | 0.05 |
| IgAN IgG+RIF Ext (pool 3) | 71 | 17.85 |
| IgAN IgG−RIF Ext (pool 7) | 157 | 44.09 |
Gd-IgA1 was used as the antigen in IgG-IgA1 immune-complex assay and IgG autoantibody assay. One unit (U) was defined as the amount of IgG resulting in optical density (OD) at 490 nm equal to 1.0. W, wash; Ext, extract from immunodeposits; IgG+RIF, IgG-positive by routine immunofluorescence (pool 3 of kidney-biopsy specimens); IgG−RIF, IgG-negative by routine immunofluorescence (pool 7 of kidney-biopsy specimens).