Table 4.
IgG isolated by the two-washes protocol from renal immunodeposits of patients with IgG−RIF IgAN but not from renal immunodeposits of patients with MN and LN is specific for Gd-IgA1
| Kidney Specimens | IgG Autoantibody (U/μg IgG) W1a | IgG Autoantibody (U/μg IgG) W2 | IgG Autoantibody (U/μg IgG) Ext |
|---|---|---|---|
| IgAN (pool 4) | 1 | 3 | 68 |
| MN | 1 | 1 | 5 |
| LN-D | 1 | 1 | 9 |
| LN-M | 0 | 2 | 6 |
Samples were from patients with IgG−RIF IgAN (n=5, pool 4), primary MN (n=3, individually processed, average values shown), LN-D (n=3, pooled), and LN-M (n=5, pooled). W1, wash 1; W2, wash 2; Ext, extracts from immunodeposits.
a
IgG autoantibody assay was used; one unit (U) of Gd-IgA1–specific IgG was defined as the amount resulting in optical density (OD) at 490 nm equal to 1.0. The results were normalized per microgram of total IgG.