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. 2019 Jul 11;30(10):1870–1885. doi: 10.1681/ASN.2018101067

Figure 5.

Figure 5.

Anti-CD45RB with or without anti–Tim-1 treatment after IRI-facilitated renal recovery after renal IRI. (A) Anti-CD45RB, anti-CD45RB with anti–Tim-1, or PBS was administered to WT mice 1 day after IRI; mice were harvested on days 3 and 5 after IRI. (B) Levels of serum creatinine and BUN after IRI. (C) Flow cytometry analysis of splenic Bregs and Tregs after IRI. (D) Flow cytometry analysis of renal leukocytes, Bregs, and Tregs after IRI. (E) Renal tubular regeneration measured by Ki67 staining on day 5. Original magnification, ×200. Results were expressed as dot plots with the mean±SEM. n=6–7 (A–D) or 3 (E). *P<0.05, **P<0.01, compared with the PBS group. #P<0.05, anti-CD45RB group compared with the anti-CD45RB/anti–Tim-1 group. Bregs, regulatory B cells; HPF, high-power field; IRI, ischemia-reperfusion injury; Tregs, regulatory T cells; WT, wild type.