Table 1.
Mean survival time of mice groups.
| Animal group | #of mice/group | Treatment | Survival time (DPI) (Mean ± SD) |
|---|---|---|---|
| Mock | 9 | - | 250 |
| Mock-AR12 | 4 | AR12 | 250 |
| Mock-Rapamycin | 4 | Rapamycin | 250 |
| RML | 9 | - | 148.11 ± 2.57 |
| RML-AR12 | 8 | AR12 | 158.12 ± 5.96 |
| RML-Rapamycin | 9* | Rapamycin | 161.83 ± 5.19 |
| RML-5RW | 7* | 5RW | 161.83 ± 4.16 |
| RML-60SH | 10 | 60SH | 173.87 ± 6.79 |
| RML-(AR12 + 5RW) | 10* | AR12 + 5RW | 162.11 ± 3.68 |
| RML-(AR12 + 5RW) | 8 | AR12 + 60HS | 165.75 ± 4.65 |
| RML-(Rapamycin+5RW) | 10 | Rapamycin+5RW | 167 ± 2.74 |
| RML-(Rapamycin+60SH) | 8 | Rapamycin+60SH | 173 ± 9.82 |
*One animal in the group did not show any prion signs until the end of the experiment and sacrificed at 250 DPI. This one animal is not considered in the calculation of mean since the calculations are only for prion sick mice.
FVB mice were either mock or RML infected. Mock groups were sacrificed at 250 days post mock i.c. inoculation.
AR-12 treatment was started 4 weeks after i.c. inoculation. The drug was I.P. injected for 4 weeks (5mg/ml) then shifted to dissolving the drug in water (50ug/ml, 15,000ug) until the end of the experiment.
Rapamycin treatment started 4 weeks after i.c. inoculation. The drug was I.P. injected for 4 weeks (20mg/ml) only.
Cellulose ethers (5RW and 60SH) were given as a single subcutaneous dose (4g/kg) 4 weeks before i.c. inoculation.