Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 24;16(10):1377–1385. doi: 10.1080/15476286.2019.1630797

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Analysis of the folding of Kt-7 induced by addition of Mg²⁺ ions, analysed by steady-state FRET. The sequence of the unmodified RNA is shown at the top; four variants in which a chosen adenine was replaced by N⁶-methyladenine were also prepared. Each species is labelled at the 5ʹ-termini by fluorescein (flu, donor) and Cy-3 (Cy3, acceptor) fluorophores. The efficiency of energy transfer (E_FRET) is plotted as a functionofMg²⁺ concentration for each species. Where possible the data have been fitted to a two-state model of RNA folding Equation (1).

(a). Unmodified (filled circles) and L3-methylated (open circles) Kt-7. (b). Kt-7 with A1n replaced by N⁶-methyladenine. This species failed to fold at any concentration of Mg²⁺. (c). Kt-7 with A2b replaced by N⁶-methyladenine. (d). Kt-7 with A3b replaced by N⁶-methyladenine.