Figure 2.
Northern blot analysis of Rli47 expression on a σB-dependent manner. Samples were taken from L. monocytogenes EGD-e wild-type (WT), ΔsigB and Δrli47 cultures at exponential and stationary phases in DM + 0.4% Glucose at 37°C. Northern blot was probed for Rli47 and 16S rRNA as a loading control. Relative levels of Rli47 transcripts were normalized to 16S and are shown below each lane. Vertical lines between bands represent a non-sequential loading of the samples of interest. Representative of two independent technical replicates of two biological samples per condition.