Figure 5.

SAL alleviated HG-induced ARPE-19 cells injury by down-regulation of miR-138.

After transfection with miR-138 mimic/NC and treatment with HG (50 mM) and SAL (3 μM), (a) cell viability, (b) cell apoptosis, (c and d) apoptosis-associated factors, (e and f) the pro-inflammatory cytokines of TNF-α and IL-6, and (g) the level of ROS in HG and SAL co-treated cells were determined by CCK-8, flow cytometry, western blot and DCFH-DA molecular probe assays. *, P < 0.05, **, P < 0.01, ***, P < 0.001.