Figure 1.

RNase catalytic activity of Regnase-1 is required for the larva-to-adult metamorphosis.

(a) Domain structures of Drosophila Regnase-1 (CG10889) and human Regnase-1. Amino acid sequence alignment of Regnase-1 orthologues around the residues mutated in Figure 1(e) is also shown. Dme, Drosophila melanogaster; Cel, Caenorhabditis elegans; Dre, Danio rerio; Hsa, Homo sapiens. (b) Knockout (KO) allele of Drosophila Regnase-1 created in this study. Nucleotide residues targeted by sgRNA is italicised. The 5-nt residues deleted are shown in green. Amino acid residues after the frameshift mutation in the KO allele are shown in magenta. (c) Life cycle of Drosophila. (d) mRNA expression levels of regnase-1, drosha, dicer-1, and RpL32, in wild-type third instar larvae and early pupae, determined by mRNA-seq. Mean ± SD for three biological replicates. (e) Representative images of third instar larvae, early pupae, dead late pupae, and eclosed adult flies, for indicated genotypes. Successful metamorphosis rates are shown with the numbers of successful metamorphosis event/the numbers of examined pupae shown in parenthesis.