Figure 3.

(a) The surrounding sequence of each selected PTCs is compared to the most favourable surrounding sequence for read-through (indicated in blue). Each dot represents identical residue to the consensus sequence. The efficacy of read-through for all PTCs tested is reported as mean ± SEM (n > 4) and calculated by using the anti-GFP antibody. For the R59X and E364X mutants, the asterisk indicates that the calculated efficiency is the cumulative effect of read-through and mRNA stabilization. (b, c, d) Representative western blot of full-length GFP-CDKL5 (α-CDKL5; black arrowheads) or truncated proteins (white arrowheads) in transfected HEK293 cells exposed to vehicle (-) or to 2000 μg/ml of G418 (+). In c and d, the loaded volume of WT samples was halved to avoid signal saturation.