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. Author manuscript; available in PMC: 2020 Sep 5.
Published in final edited form as: Mol Cell. 2019 Aug 27;75(5):1007–1019.e5. doi: 10.1016/j.molcel.2019.07.024

Figure 2. Hairpin opening occurs during forward 30S head rotation.

Figure 2.

(A) Diagram of predicted EF-G residence times in presence of antibiotic fusidic acid for unwinding resulting from either forward or reverse 30S head rotation.

(B, C) An example of a single-ribosome ‘fleezers’ trajectory in presence of 50–200 µM fusidic acid and 10 nM Cy3-EF-G. Hairpin is held under high external force of 13–16 pN. (C) Optical tweezers channel, data was recorded at 133 Hz and displayed at 13 Hz.

(D) Fluorescence channel, data is recorded at 100 Hz and displayed at 10 Hz. Additional zoomed-in events shown in Figure S3. (D) Summary of average EF-G residence times before and after unwinding show that τrelease increases dramatically in the presence of fusidic acid (n = 25 events, 13 molecules). Error bars represent SEM.