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. Author manuscript; available in PMC: 2020 Sep 5.
Published in final edited form as: Mol Cell. 2019 Aug 27;75(5):1007–1019.e5. doi: 10.1016/j.molcel.2019.07.024

Figure 5. Novel ribosome-dependent hairpin dynamics.

Figure 5.

(A-F) Examples of single-ribosome ‘fleezers’ trajectories in presence of 10 nM Cy3-labeled-EF-G where the unwinding step is split into two sub-steps that add up to one codon. Notice that EF-G remains bound until the entire step is completed. For comparison, a second unwinding step that occurs in a single transition is shown in A. The sub-steps are highlighted with red arrows.

(G) An example of a single-ribosome ‘fleezers’ trajectory in presence of 10 nM Cy3-labeled-EF-G showing a region of hairpin hopping where several EF-G molecules unproductively bind for long time scales (shown with stars).