(A-F) Examples of single-ribosome ‘fleezers’ trajectories in presence of 10 nM Cy3-labeled-EF-G where the unwinding step is split into two sub-steps that add up to one codon. Notice that EF-G remains bound until the entire step is completed. For comparison, a second unwinding step that occurs in a single transition is shown in A. The sub-steps are highlighted with red arrows.
(G) An example of a single-ribosome ‘fleezers’ trajectory in presence of 10 nM Cy3-labeled-EF-G showing a region of hairpin hopping where several EF-G molecules unproductively bind for long time scales (shown with stars).