Figure 5.
A, two-dimensional 1H-1H trNOESY spectra of VG16KRKP–KYE28 in a 3:1 POPE/POPG vesicle, displaying the NOE contacts in the fingerprint region for CαH-NH and NH-NH resonances important for structure stabilization. VG16KRKP–KYE28 upon interaction with 3:1 POPE/POPG vesicles exhibited several trNOEs hinting toward the adoption of a rigid and well-converged structure. B, parent peptide complex, also displayed intra- and inter-molecular long-range NOE contacts. The experiments were performed by titrating 0.6 mm of the total peptides with 12 μm of 3:1 POPE/POPG LUV (LUV: each peptide = 1:25) at pH 4.5, using a Bruker Avance III 700 MHz NMR spectrometer (NOESY mixing time = 150 ms) and at 298 K (residues marked in blue are from VG16KKRP and in red from KYE28).