Figure 5.

Domain prediction for G. gallus EDIL3 and MFGE8 protein sequences. GenBank^TM accession numbers used are EDIL3 XP424906.3 and MFGE8 NP_001264039.1. Alignment was performed using Clustal Omega method in MEGA7 and then annotated with BioEdit. Red- and blue-colored residues indicate identity and similarity, respectively. Blue and yellow backgrounds correspond to EGF-like and F5/8C domains, respectively. Red vertical arrows (filled for cysteines) and green circles correspond to amino acid residues involved in Ca²⁺ binding and PS binding, respectively. The letters below arrows and circles indicate the consensus pattern for Ca²⁺-binding and PS-binding residues of human coagulation factors IX/X and mouse MFGE8, respectively. Indicated positions correspond to the residue numbering for the full-length sequences of G. gallus EDIL3 and MFGE8.