Figure 2.

Pheromone-induced vacuolar targeting is mediated by the PI 3-kinase complex II and the MAPK Fus3. Time course of vacuolar targeting induced by 1 μm α-factor in BY4741 bar1Δ cells lacking Vps34 (A), Vps38 or Atg14 (B), and Fus3 or Kss1 (C). D, flow cytometry analysis of organelle abundance in WT and vps34Δ cells. Abundance is reported by fluorescence from GFP fused with Chc1 (late Golgi/clathrin), Cop1 (early Golgi), Sac6 (Actin cytoskeleton), or Sec13 (endoplasmic reticulum-to-Golgi transport vesicles). Rosella experiments were done at least two times, and data are mean ± S.D. from one experiment. Flow cytometry data are ± S.D. for four biological replicates (10,000 cells each).