Figure 5.

F-box protein Pfu1 is required for proper morphogenesis. A, F-box screen. Bars represent average mating projection lengths relative to WT BY4741 (WT) (n = 200 cells). Cells were treated with 30 μm α-factor for 18 h prior to imaging. B, live cell imaging of the polarity marker Bem1–GFP in cells treated with 300 nm α-factor for 18 h. Shown is a schematic of the microfluidics device as well as representative images and kymographs of Bem1–GFP fluorescence. C, β-gal activity in cells expressing the FUS1-lacZ transcription reporter and treated with 0–3 μm α-factor for 90 min. Data represent percentage of maximum activity in WT cells. All data are ± S.D.