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. 2019 Aug 8;294(40):14674–14685. doi: 10.1074/jbc.RA118.006588

Figure 5.

Figure 5.

SLFN11 mutant–expressing stable cell lines are differentially sensitized upon DNA damage. a, stable expression of SLFN11 mutants in HEK293/SLFN11KO cell lines as created by lentiviral infection. b, relative viability of stable SLFN11 mutant–expressing HEK293 cell lines was measured by MTS assay after 48 h of 200 nm CPT or DMSO treatment (biological replicates, mean ± S.D. (error bars), n = 3; **, p < 0.01; ***, p < 0.001). c, microscopic images at 10× magnification of stable SLFN11 mutant–expressing HEK293 cells after 48 h of 200 nm CPT or DMSO treatment. d, total RNA from stable SLFN11 mutant–expressing HEK293 cell lines treated for 48 h with 200 nm CPT or DMSO was visualized by SYBR Gold staining, and the presence of the respective proteins was determined by immunoblotting. e, relative type II tRNA levels from samples shown in d were quantified and normalized to 5.8S rRNA (biological replicates, mean ± S.D. (error bars), n = 3; *, p < 0.05; ***, p < 0.001).