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Position 32 is modified to m5Cm in T. kodakarensis tRNATrp. Top, extracted ion chromatography (XIC) showing coelution of the nucleoside modified at position 32 in tRNATrp from T. kodakaraensis and m5Cm in human cytoplasmic tRNALeuCAA from ALKBH1 knockout cells. Bottom, CID spectrum of m5Cm. The cleavage position of the base-related ion is indicated on the chemical structures.
