TABLE 1.
Germination properties of B. licheniformis wild-type and mutant strainsa
| Strain | Genotype | Protein(s) present | Mean no. of spores by microscopic count ± SDb |
Mean Gmax ± SDc |
||||
|---|---|---|---|---|---|---|---|---|
| l-Ala | l-Cys | l-Val | l-Ala | l-Cys | l-Val | |||
| WTd | Ynd, GerA, GerK | 96 ± 1 | 97 ± 1 | 78 ± 13 | −1.5 ± 0.3 | −1.9 ± 0.4 | −0.7 ± 0.2 | |
| NVH-1387 | ΔyndDE3E2F1E1 | GerA, GerK | 47 ± 7 | 36 ± 10 | 52 ± 5 | −0.4 ± 0.2 | −0.1 ± 0.0 | −0.4 ± .0.1 |
| NVH-1412 | ΔyndDE3E2F1E1 ΔgerKACB | GerA | 46 ± 2 | 39 ± 0 | 47 ± 2 | −0.3 ± 0.1 | −0.2 ± 0.0 | −0.3 ± .0.0 |
| NVH-1389 | ΔgerAB | Ynd, GerK, GerAA, GerAC | 2 ± 2 | 4 ± 4 | 2 ± 1 | −0.1 ± 0.0 | −0.1 ± 0.0 | −0.1 ± .0.0 |
| NVH-1369 | ΔyndE3 | YndDE2F1E1, GerA, GerK | 58 ± 6 | 48 ± 8 | 57 ± 1 | −0.4 ± 0.0 | −0.4 ± 0.0 | −0.4 ± .0.0 |
| NVH-1378 | ΔyndE3E2 | YnDF1E1, GerA, GerK | 50 ± 4 | 41 ± 3 | 53 ± 6 | −0.5 ± 0.1 | −0.4 ± 0.2 | −0.5 ± .0.2 |
| NVH-1405 | ΔyndE2E3E1 | YndDF1, GerA, GerK | 86 ± 5 | 76 ± 7 | 85 ± 5 | −0.9 ± 0.3 | −0.6 ± 0.1 | −0.8 ± .0.2 |
| NVH-1371 | ΔyndF2 | Ynd, GerA, GerK | 95 ± 4 | 98 ± 1 | 70 ± 12 | −1.5 ± 0.4 | −1.8 ± 0.2 | −0.5 ± .0.2 |
| NVH-1404 | NVH-1387 ΔgerKA-C | GerA, GerKB | 47 ± 5 | 42 ± 8 | 55 ± 3 | −0.4 ± 0.1 | −0.3 ± 0.1 | −0.4 ± .0.1 |
| Complementations | ||||||||
| cis | ||||||||
| NVH-1421 | NVH-1369::yndE3 | 95 ± 2 | 97 ± 1 | 75 ± 24 | −1.3 ± 0.2 | −1.3 ± 0.3 | −0.7 ± .0.1 | |
| NVH-1416 | NVH-1405::yndE3E2 | 85 ± 6 | 81 ± 4 | 80 ± 5 | −0.7 ± 0.0 | −0.6 ± 0.0 | −0.6 ± 0.1 | |
| NVH-1417 | NVH-1405::yndE3E2E1 | 95 ± 4 | 94 ± 4 | 89 ± 6 | −1.4 ± 0.3 | −1.0 ± 0.0 | −0.9 ± .0.0 | |
| NVH-1413 | NVH-1404::yndDE2F1 | 47 ± 5 | 48 ± 5 | 37 ± 3 | −0.5 ± 0.0 | −0.4 ± 0.0 | −0.3 ± .0.0 | |
| NVH-1427 | NVH-1412::yndDF1 | 69 ± 6 | 60 ± 3 | 71 ± 4 | −0.4 ± 0.0 | −0.3 ± 0.0 | −0.4 ± 0.0 | |
| trans | ||||||||
| NVH-1474 | NVH-1412/pHT315 | 28 ± 5 | 14 ± 6 | 27 ± 1 | −0.2 ± 0.0 | −0.1 ± 0.0 | −0.2 ± .0.0 | |
| NVH-1438 | NVH-1412/pHT315-yndDF1 | 49 ± 9 | 36 ± 11 | 55 ± 10 | −0.4 ± 0.1 | −0.2 ± 0.1 | −0.4 ± .0.1 | |
| NVH-1473 | NVH-1412/pHT315-yndD | 24 ± 3 | 14 ± 4 | 27 ± 4 | −0.2 ± 0.0 | −0.1 ± 0.0 | −0.2 ± .0.0 | |
a
All data are presented as means from three biological replications.
b
The percentages of germinated (phase-dark) spores were determined after 120 min of exposure to 100 mM germinant compounds.
c
Gmax is the maximum rate of germination (ΔOD600 units per minute).
d
The wild-type (WT) strain shows 1% ± 1% germination with just buffer (no germinants added), as determined by microscopic counts.