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Cyclic di-GMP positively regulates tfoY transcription. P1-tfoY transcriptional reporter (pBRP37) activity was measured in high (DGC), unaltered (DGCMUT and PDEMUT), and low (PDE) levels of cyclic di-GMP. Data are averages and standard deviations from three biological replicates. Bars with different letters indicate statistically significant differences determined by one-way analysis of variance followed by Tukey’s multiple-comparison test (P < 0.05).
