FIG 5.
Immunodetection of the T2SS Xcp machinery component XcpP in cellular extracts. Shown is immunodetection of XcpP at 25 kDa after 2, 3, 4, 5, 6, 7, and 24 h of growth of indicated strains at 37°C with aeration. Glucose and arabinose were added in the culture medium after 1 h 30 min of growth at a final concentration of 0.5%, allowing, respectively, repression or induction of the PBAD promoter of pTS51 bearing qslA. In order to standardize the protein samples, the equivalent of 0.1 OD600 unit was loaded.