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Cytotoxicity toward J774 macrophages evaluated using an LDH assay after 2 h of infection with PAO1 (WT and qslA mutant) and after 45 min of infection with PA14 (WT and QslA overproduction). Arabinose at 0.5% was added in the culture medium after 1 h 30 min of growth to induce the PBAD promoter of pTS51 bearing qslA. Error bars correspond to SDs from three independent experiments done in triplicate. No statistical differences were observed (P values calculated with Student’s t test).
