Overall shape		Heart shape			Beating	Endothelial problems
	normal	abnormal	normal	Situs inversus	edema	Normal or abnormal	Blood flow in ISV
piezo1+/+	10/10	0/10	10/10	0/10	0/10	7/7 normal	Normal vasculature See Shmukler et al., 2015 See Shmukler et al., 2016 See Kok et al., 2016
piezo1-/-	11/14	3/14 (tail bent up)	13/14	0/14	1/14	12/12 normal
trpp2+/+, +/-	18/18	0/18	16/18	2/18	0/18	18/18 normal	Normal vasculature See Goetz et al., 2014 Schottenfeld J et al., 2007
trpp2-/-	0/16	16/16 (tail bent up)	10/16	6/16	0/16	10/10 normal
trpv4+/+	4/6	2/6 (tail bent up)	7/7	0/7	0/7	7/7 normal	Normal vasculature (4/4)
trpv4-/-	4/8	4/8 (tail bent up)	6/8	2/8	0/8	4/7 normal 3/7 slow	Normal vasculature no flow (2/4)
notch1b+/+	10/10	0/10	9/10	0/10	1/10	10/10 normal	Normal vasculature (21/22)
notch1b-/-	10/10	0/10	25/26	1/26	0/26	10/10 normal	Normal vasculature (5/6)
klf2a+/+	50/50	0/50	10/10	0/10	0/10	10/10 normal	Normal vasculature (5/5)
klf2a-/-	10/10	0/10	10/21	1/21	11/21	9/10 normal 1/10 slow	Normal vasculature (4/5) 1/5 no flow (1/5)
tnnt2aMO normal	17/17	0/17	15/17	2/17	0/17	10/10 normal	Normal vasculature (5/5)
tnnt2aMO slow	7/15	8/15 (tail bent up)	0/15	0/15	15/15	6/6 slow	no flow (5/5)
yap1+/+	17/17	0/17	17/17	0/17	0/17	17/17 normal	Subtle vascular defects See Nakajima et al., 2017
yap1-/-	27/28	1/28 (tail bent up)	25/28	0/28	3/28	26/28 normal, 2/28 no beating