Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | Mouse: Piezo1f/f(Piezo1tm2.1Apat/J) | Jackson Laboratories | JAX: 029213; RRID:IMSR_JAX:029213 | |
| Genetic reagent (M. musculus) | Mouse: Dmp1-Cre | Bivi et al., 2012 | N/A | |
| Genetic reagent (M. musculus) | Mouse: Yap1f/f;Tazf/f | Xin et al., 2013 | N/A | |
| Genetic reagent (M. musculus) | Mouse: WT C57BL/6J | Jackson Laboratories | JAX: 000664; RRID:IMSR_JAX:000664 | |
| Commercial assay or kit | Mouse Osteocalcin Immunoassay Kit | Thermo Fisher | Cat# J64239 | |
| Commercial assay or kit | Fluo-8 Calcium Flux Assay Kit | Abcam | Cat# ab112129 | |
| Commercial assay or kit | RatLaps (CTX-I) EIA kit | Immunodiagnostic Systems | Cat# AC-06F1 | |
| Commercial assay or kit | TruSeq stranded mRNA kit | Illumina | Cat# 20020594 | |
| Commercial assay or kit | High-capacity cDNA reverse transcription kit | Life Technologies | Cat# 4368813 | |
| Commercial assay or kit | RNeasy mini kit | QIAGEN | Cat# 74106 | |
| Cell line (Murine) | 293T | ATCC | CRL-3216 | |
| Cell line (Murine) | MLO-Y4 | Kato et al., 1997 | ||
| Cell line(Murine) | UAMS-32 | O'Brien et al., 1999 | Cell line maintained in Charles O’Brien lab | |
| Transfected construct (M. musculus) | Piezo1 shRNA forward | Zhang et al., 2017 | Oligo | CCGGTCGGCGCTTGCTAGAACTTCACTCGAGTGAAGTTCTAGCAAGCGCCGATTTTTG |
| Transfected construct (M. musculus) | Piezo1 shRNA reverse | Zhang et al., 2017 | Oligo | AATTCAAAAATCGGCGCTTGCTAGAACTTCACTCGAGTGAAGTTCTAGCAAGCGCCGA |
| Transfected construct (M. musculus) | Yap1 shRNA | Sigma-Aldrich | TRCN0000238432 | |
| Transfected construct (M. musculus) | Taz shRNA | Sigma-Aldrich | TRCN0000095951 | |
| Sequenced-based reagent | Piezo1 | Life Technologies | Mm01241549_m1 | |
| Sequenced-based reagent | Piezo2 | Life Technologies | Mm01265861_m1 | |
| Sequenced-based reagent | Ptgs2 | Life Technologies | Mm00478374_m1 | |
| Sequenced-based reagent | Cyr61 | Life Technologies | Mm00487498_m1 | |
| Sequenced-based reagent | Wnt1 | Life Technologies | Mm01300555_g1 | |
| Sequenced-based reagent | Yap1 | Life Technologies | Mm01143263_m1 | |
| Sequence-based reagent | Taz | Life Technologies | Mm01289583_m1 | |
| Sequence-based reagent | Tnfsf11 | Life Technologies | Mm00441906_m1 | |
| Sequence-based reagent | Tnfrsf11b | Life Technologies | Mm00435452_m1 | |
| Sequence-based reagent | Sost | Life Technologies | Mm00470479_m1 | |
| Sequence-based reagent | Mrps2 | Life Technologies | Mm00475529_m1 | |
| Sequence-based reagent | Piezo1 sgRNA | This paper | GGTTATTCCTGTGAGGCCCG | |
| Sequence-based reagent | Piezo1 sgRNA | This paper | TTAGGATTCGGCTCACAGAG | |
| Chemical compound, drug | Yoda1 | Sigma-Aldrich | Cat# SML1558 | |
| Chemical compound, drug | Puromycin dihydrochloride | Sigma-Aldrich | Cat# P8833 | |
| Chemical compound, drug | G418 disulfate | Sigma-Aldrich | Cat# G8168 | |
| Antibody | YAP1 | Cell Signaling | Cat# 14074S; RRID:AB_2650491 | 1:200 |
| Antibody | Goat anti-Rabbit IgG (Alexa Fluor 488) | Abcam | Cat# ab150077; RRID:AB_2630356 | 1:200 |
| Software, algorithm | Prism 8 | GraphPad | https://www.graphpad.com/scientific-software/prism/ | |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij |