Fig. 4.

(A) Effect of Esc(1-21) and Esc(1-21)-1c at 1/8 MIC on the swimming, swarming or twitching motility of P. aeruginosa AA11. Bacterial cells were inoculated in 0.3 %, 0.5% or 1% agarose plates (for swimming, swarming or twitching motility assays, respectively). Swimming, swarming and twitching zones were determined after 24 h of incubation at 30 °C. Control samples (Ctrl) were plates inoculated with bacteria in the absence of peptide. All experiments were performed in triplicates with 3 technical repeats and statistical significance was determined using the Student’s t test. Data are shown as mean ± SEM. **, p<0.01; ****, p<0.0001. (B): Representative images of swimming, swarming and twitching activity of P. aeruginosa in the presence of each peptide compared to Ctrl, 48 h after incubation at 30 °C. Dotted circle line indicates the zonal extent of bacterial growth. Images are at 1x magnification. All scale bars represent 0.5 cm.