Fig. 5.

Effect of Esc peptides on the expression of the following genes in P. aeruginosa AA11: fleN, flagellar synthesis regulator fleN; fimT, type IV fimbrial biogenesis protein FimT; fimX, twitching motility regulating phosphodiesterase; lasI, autoinducer synthesis protein LasI; lasB, elastase LasB; rhlA, rhamnosyltransferase chain A; rhlB, rhamnosyltransferase chain B; rpoS sigma factor RpoS; fliC, flagellin type B. Ten mL of bacterial planktonic cells culture at a density of 10⁸ CFU/mL was incubated with 1 μM peptide for 20 h in an orbital shaker at 37 °C. Total RNA was extracted immediately after the incubation. Results are representative of three independent experiments. Data are shown as mean ± SEM. Statistical analyses were performed by one-way ANOVA and Dunnett’s multiple comparisons test. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001 compared to untreated control cells, Ctrl (otherwise non-significant).