Table 1.

SnRK1-KD expression inhibits polysome formation. Indicated strains were grown in the absence or presence of CuSO₄ to induce kinase expression, and polysome profiles obtained and quantified as described in Fig. 7. Average polysome:non-polysome ratios (P:NP) are displayed along with standard error of the mean (SEM), number of independent replicates (n), and % reduction in P:NP ratio between the uninduced and induced samples.

Strain	Copper Induction	P:NP Ratio	SEM	n	% Reduction
Jo55-At 4E-KD	−	1.39	0.15	6	50.38
Jo55-At 4E-KD	+	0.69	0.08	5
Jo55-At 4E-KDKR	−	1.27	0.05	2	17.32
Jo55-At 4E-KDKR	+	1.05	0.11	2
Jo55-Hs 4E-KD	−	1.72	0.06	2	18.60
Jo55-Hs 4E-KD	+	1.40	0.06	2
Jo55-At 4E-VA-KD	−	0.97	0.04	2	6.19
Jo55-At 4E-VA-KD	+	0.91	0.04	2
Jo55-At iso4E-KD	−	0.85	N/A	1	57.65
Jo55-At iso4E-KD	+	0.36	N/A	1
Jo55-At iso4E-KDKR	−	0.98	N/A	1	34.69
Jo55-At iso4E-KDKR	+	0.64	N/A	1

Indicated strains were grown in the absence or presence of CuSO₄ to induce kinase expression, and polysome profiles obtained and quantified as described in Fig. 7. Average polysome:non-polysome ratios (P:NP) are displayed along with standard error of the mean (SEM), number of independent replicates (n), and % reduction in P:NP ratio between the uninduced and induced samples.