Table 1.
Statistical analyses of the main effect of treatment on protein abundance or phosphorylation (parametric ANOVA or Kruskal-Wallis ANOVA in ethanol-naïve, non-dependent, and dependent rats), correlation of protein abundance or phosphorylation with the number of ethanol reinforcers earned during the last self-administration session (Pearson or Spearman test in non-dependent and dependent rats only), and correlation of protein abundance or phosphorylation with the blood ethanol concentrations (BECs) measured during the last period of ethanol vapor inhalation prior to brain collection (Pearson or Spearman test in dependent rats only). Pearson and Spearman correlation coefficients (RP and RS, respectively) are shown as absolute value. p/t, phosphorylated protein over total protein ratio; p-CSM, phosphorylated CDK substrate motif.
| Brain region | Protein | Main effect of treatment on protein abundance or phosphorylation | Correlation with ethanol intake | Correlation with final BECs |
|---|---|---|---|---|
| BNST | CDK5 | F2,30=2.1, n.s. | RP =0.3, n.s. | RP=0.7, p<0.05 |
| p35 | H2=0.3, n.s. | RS=0.2, n.s. | RS=0.01, n.s. | |
| DARPP32 (p/t) | H2=0.4, n.s. | RS=0.06, n.s. | RS=0.2, n.s. | |
| PSD95 (p/t) | H2=2.3, n.s. | RS=0.4, n.s. | RS=0.3, n.s. | |
| PAK1 (p/t) | F2,30=2.8, n.s. | RP =0.2, n.s. | RP=0.8, p<0.01 | |
| P27Kip1 (p/t) | F2,30=1.4, n.s. | RP =0.02, n.s. | RP=0.4, n.s. | |
| p-CSM (150 kDa) | F2,28=0.1, n.s. | RP =0.01, n.s. | RP=0.6, n.s. | |
| p-CSM (37–75 kDa) | F2,29=0.3, n.s. | RP =0.06, n.s. | RP=0.5, n.s. | |
| CeA | CDK5 | H2=1.7, n.s. | RS=0.2, n.s. | RS=0.3, n.s. |
| p35 | F2,29=1.6, n.s. | RP=0.2, n.s. | RP=0.05, n.s. | |
| p-CSM (150 kDa) | F2,29=0.2, n.s. | RP =0.2, n.s. | RP=0.3, n.s. | |
| p-CSM (37–75 kDa) | H2=1.4, n.s. | RS=0.2, n.s. | RS=0.4, n.s. | |
| BLA | CDK5 | H2=1.6, n.s. | RS=0.006, n.s. | RS=0.06, n.s. |
| p35 | H2=2.1, n.s. | RS=0.2, n.s. | RS=0.1, n.s. | |
| p-CSM (150 kDa) | H2=6.6, p<0.05 | RS=0.5, n.s. | RS=0.04, n.s. | |
| p-CSM (37–75 kDa) | F2,18=3.9, p<0.05 | RP =0.3, n.s. | RP=0.5, n.s. | |
| Acb | CDK5 | F2,30=0.1, n.s. | RP=0.1, n.s. | RP=0.08, n.s. |
| p35 | F2,30=0.1, n.s. | RP=0.08, n.s. | RP=0.1, n.s. | |
| p-CSM (150 kDa) | F2,29=1.4, n.s. | RP =0.02, n.s. | RP=0.4, n.s. | |
| Cg | CDK5 | F2,29=1.0, n.s. | RP=0.05, n.s. | RP=0.5, n.s. |
| p35 | F2,27=0.01, n.s. | RP=0.002, n.s. | RP=0.1, n.s. | |
| p-CSM (150 kDa) | F2,17=0.6, n.s. | RP =0.1, n.s. | RP=0.8, n.s. | |
| mPFC | CDK5 | F2,29=0.6, n.s. | RP=0.05, n.s. | RP=0.3, n.s. |
| p35 | F2,29=0.7, n.s. | RP=0.3, n.s. | RP=0.2, n.s. | |
| p-CSM (150 kDa) | F2,19=0.4, n.s. | RP =0.2, n.s. | RP=0.5, n.s. | |
| OFC | CDK5 | F2,30=0.6, n.s. | RP=0.08, n.s. | RP=0.2, n.s. |
| p35 | F2,30=0.5, n.s. | RP=0.2, n.s. | RP=0.3, n.s. | |
| p-CSM (150 kDa) | F2,28=0.6, n.s. | RP =0.1, n.s. | RP=0.3, n.s. |