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EVs纯化方法优缺点比较

Advantages and disadvantages of current approaches to EV isolation

Methods	Advantages	Disadvantages
Successive differential centrifugation	High purity	Highly labour intensive, limited processing capacity, low yield and time consuming
Size-exclusion	Easy manipulation and relatively high purity	limited processing capacity and low yield
Immunomagnetic sorting,	High specificity and high purity	Low yield and there is no molecule specific to exosome
Precipitation reagents	Convenience and high yield	Susceptible to the contamination of precipitated molecules