TABLE 2.
MS/MS parameters for detection of de novo formed sphingolipids using the TQ MS system.
| Collision | Dwell | ||
| Sphingolipid species | Mass transition (m/z) | energy (eV) | time (ms) |
| 3KS-d5 | 305.3 → 273.3 | 19 | 160 |
| d18:0 Sph-d5 | 307.3 → 289.3 | 15 | 160 |
| Cer d18:0-d5/16:0-d3 | 548.6 → 530.6 | 15 | 160 |
| 548.6 → 289.3 | 25 | 160 | |
| Cer d18:1-d5/16:0-d3 | 528.6 → 269.3 | 25 | 160 |
| REFERENCE COMPOUNDS | |||
| d17:0 Sph (IS)a | 288.5 → 270.5 | 12 | 160 |
| Cer d18:1/17:0 (IS)b | 534.5 → 264.3 | 25 | 160 |
| d18:0 Sph | 302.3 → 284.3 | 15 | 160 |
| Cer d18:0/16:0 | 540.5 → 522.5 | 15 | 160 |
| 540.5 → 284.3 | 25 | 160 | |
| Cer d18:1/16:0 | 520.5 → 264.3 | 25 | 160 |
aUsed as internal standard (IS) for labeled 3KS and d18:0 Sph. bUsed as IS for labeled C16 dhCer and C16 Cer.