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. 2019 Oct 7;9:14373. doi: 10.1038/s41598-019-50741-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Acute effect of TiNP on testicular function. Male C57BL/6J mice (n = 5) were injected with TiNP (2, 10, 50 mg/kg; i.v.) once a week for 4 weeks, and were sacrificed at 3 days (a); 9 days (b); or 90 days (c) after the final injection. Control mice received DSP (0.1 ml/20 g body weight). Mice received single IT injection of TiNPs (20, 100, or 500 μg/mouse), and were sacrificed 3 days later (d). Sperm motility (motility percent) and sperm numbers (in cauda epididymis) are shown. Results from (a) was derived from a previous paper. In two experiments (b,c), C57BL gpt delta mice were used. *, **, significantly different from control (*p < 0.05; **p < 0.01).