Fig. 3. Naringenin inhibits inflammation in GDM mice.

Relative expression levels of IL-1β, IL-6, TNF-α, and MCP-1 in mice blood (a) and SM (b) of different groups. SM, skeletal muscle; IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor α; MCP-1, monocyte chemotactic protein 1. n = 7–12 for each group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with wild-type group. *p < 0.05, **p < 0.01 between the comparison of GDM group and GDM + NAR group. c Total cell lysates were extracted, and the amount of TLR2, TLR4, phospho-NF-κB p65, phospho-JNK and the corresponding total protein levels were analyzed by Western blotting. β-actin served as a loading control. Shown are representative results from three independent experiments. d Signals of proteins were normalized against β-actin and quantified and are presented as fold change of GDM groups vs wild-type group. Shown are means ± SD of three independent experiments. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 compared with wild-type group. *p < 0.05, **p < 0.01 between the comparison of GDM group and GDM + NAR group