Fig. 2. Rev-erbα inhibits glycolysis in human gastric carcinoma cells.

a Rev-erbα siRNA was transfected into SGC-7901 cells for 48 h and Western blot was used to detect Rev-erbα protein levels. b The extracellular acidification rate (ECAR) of Rev-erbα siRNA transfected SGC-7901 was detected by Seahorse XF Analyzer, and normalized into cell number. c Both SGC-7901 and BGC-823 cells were treated with SR8278 (1–3 μM) for 48 h, and intracellular lactate was measured using a lactic acid test kit. d Cells were treated with GSK4112 (0.5–2 μM) for 48 h, and the glucose level in the cell supernatant was measured using a glucose test kit. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. Scramble siRNA or vehicle group. Mean ± SEM, N = 4–6