Fig. 3. Rev-erbα inhibits proliferation of human gastric carcinoma cells by downstream PFKFB3 and G6PD signaling.

EdU incorporation into DNA synthesis (cell proliferation) was detected using a Click-iT EdU cell proliferation assay kit. a SGC-7901 cells were transfected with scramble and Rev-erbα siRNA, and then treated with 3PO (25 μM) and DHEA (250 μM) for 48 h. b SGC-7901 cells were overexpressed with PFKFB3 and G6PD and treated with GSK4112 (2 μM) for 48 h. (Left panel) Western blot was used to detect PFKFB3 and G6PD protein levels. (Right panel) Cell proliferation assay. ^***P < 0.001 vs. Scramble plasmids. ^†P < 0.05, ^†††P < 0.001 vs. siRNA/vehicle; ^‡‡P < 0.01 vs. siRNA/3PO or siRNA/DHEA. Mean ± SEM, N = 4–5