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. 2019 Oct 7;9:14400. doi: 10.1038/s41598-019-50529-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Renal biopsy morphometry. (A) The cortical area was measured by outlining the circumference of the cortical sample in the biopsy (blue dashed line). The number of non-sclerotic glomeruli in the cortex was counted (orange arrows). Periodic acid–methenamine silver staining, original magnification × 50. (B) Glomerular tuft areas were outlined (green dotted line). The mean area of all non-sclerotic glomerular tufts in the biopsy was used to calculate mean glomerular volume. Periodic acid–methenamine silver staining, original magnification × 400.