Fig. 3. Upregulation of c-Myc promotes transactivation of GP73.

a The products of ChIP-PCR in the input, IgG and ChIP groups were analyzed using agarose gel electrophoresis. The abundance of DNA fragments was measured using ImageJ and is represented using AOD. b Luciferase activity was measured after HepG2 cells were transfected with pGL4.19 containing promoter of GOLM1/binding site mutated mutant, pRL-TK and vector/c-Myc. Data in a and b are the mean ± s.e.m., and the data are representative of three independent experiments. A two-tailed Student’s t-test was used for statistical calculation