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. 2019 Oct 7;8(10):58. doi: 10.1038/s41389-019-0166-7

Fig. 6. GP73 mediates the trafficking of intracellular MMP-7 and induces cell invasion.

Fig. 6

a 293 T cells were transfected with 2 μg pCMV-c-FLAG vector containing fragments of GP73 truncated mutants. Forty-eight hours after transfection, GP73 mutants and interacting proteins were co-immunoprecipitated with FLAG-tagged antibodies and blotted with the indicated antibodies. b 293 T cells were transfected with 1 μg pcDNA3.1-GP73-GFP and 1 μg pcDNA3.1-MMP-7-RFP vectors. Movements of GP73 (green) and MMP-7 (red) were captured every 5 s for 5 min (scale bar: 5 μm). c Overview of the GP73-mediated cell invasion process in a mildly hypoxic microenvironment: c-Myc transactivates GP73 and promotes metastasis of hepatocellular carcinoma cells through GP73-mediated MMP-7 trafficking in a mildly hypoxic microenvironment