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. 2019 Oct 7;9:14363. doi: 10.1038/s41598-019-50915-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D random motility of endothelial cells in collagen I gel. (a) Representative cell trajectories observed in two parallel HUVEC cultures – transfected with control-siRNA (green) or Tks4-siRNA (red) – are superimposed for comparison. Trajectories were extracted from 24 h long time-lapse recordings, also see Supplementary Video S4. (b) Time-dependent cell speeds, averaged over the tracked cells for each time point. (c) Average cell displacements during various time intervals. Error stripes correspond to SEM. Data from n = 112 control-siRNA cells and n = 104 Tks4-siRNA cells were collected from two independent sets of experiments.