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. 2019 Oct 7;9:14363. doi: 10.1038/s41598-019-50915-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chorion vessel analysis. Representative fluorescent microscopic images of chorions from (a) WT or (b) Tks4-KO embryos where endothelial cells were visualized by CD34 immunofluorescent labeling (green), scale bar: 100 μm. Quantitative analysis of vessel parameters in 3 WT and 3 Tks4-KO individuals: (c) vessel density, (d) average width of vessels, (e) total length of all vessels in a 865 μm × 650 μm microscopic field. Error bars correspond to SEM and asterisks (*) indicate statistically significant differences with Student’s t-test, $p < 0.05$ .