Skip to main content
. 2019 Oct 1;9:752. doi: 10.3389/fonc.2019.00752

Figure 2.

Figure 2

Magnolol regulates the expression of EMT marker genes in human colon cancer cells. (A) HCT116 and SW480 cells were treated with 0, 2.5, 5, or 10 μM magnolol for 24 h, and western blots were performed for E-cadherin, ZO-1, Claudin, and α-tubulin (used as an internal control). (B) HCT116 cells were treated with 0, 2.5, 5, or 10 μM magnolol for 24 h, and western blots were conducted for N-cadherin, TWIST1, Slug, Snail, and α-tubulin. (C) mRNA expression of E-cadherin, ZO-a, and Claudin in HCT116 cells treated with magnolol (0, 2.5, 5, or 10 μM) for 24 h. (D) mRNA expression of N-cadherin, TWIST1, Slug, and Snail in HCT116 cells treated with magnolol (0, 2.5, 5, or 10 μM) for 24 h. In (C,D), GAPDH served as a control. All data values labeled with different letters are significantly different (p < 0.05).