Figure 5.

Zinc chelation prior to DENV infection inhibits DENV infection. (A) Caco-2 or A549 cells were treated with DMSO or TPEN (0.5 μM) for 4 h prior to DENV infection. Infected cells were cultured for 24 h in the absence of TPEN and viral titers in the supernatant was measured by plaque assay and (B) DENV RNA was quantitated by qRT-PCR. (C) Cells pre-treated with TPEN and infected with DENV were collected by trypsinization 1 h pi and total RNA was isolated to measure internalized viral RNA by qRT-PCR. (D) Caco-2 cells were treated with DMSO or TPEN (0.5 μM) for 4 h prior to RV infection. Infected cells were cultured for 16 h in the absence of TPEN and viral titers in the supernatant was measured by plaque assay. All the data are from at least two independent experiments with triplicate samples. Data are presented as mean ± SD. **p < 0.01.