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. 2019 Oct 1;10:2347. doi: 10.3389/fimmu.2019.02347

Figure 7.

Figure 7

Zinc chelation induces antiviral response. (A) Inset: Caco-2 cells were treated with 0.5 μM TPEN and DMSO for 4 and 8 h and used for western blot analysis of HSP70. Caco-2 cells were treated with DMSO or TPEN (0.5 μM) in the presence of 50 μM Salubrinal after infection and virus titers were determined at 24 h pi. (B) Caco-2 cells were treated with DMSO or TPEN (0.5 μM) for 4 or 8 h. Nuclear extracts were prepared and NF-κB DNA binding activity was assessed by EMSA. Oct1 served as a control for normalization. (C–G) Caco-2 cells were treated with DMSO or TPEN (0.5 μM) for 4 or 8 h. Total RNA was isolated and transcript levels of indicated genes were quantitated by RT-PCR. All the data are from at least two independent experiments with triplicate samples. Data are presented as mean ± SD. **p < 0.01, ***p < 0.001, ****p < 0.0001.