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. 2019 Oct 7;9:14376. doi: 10.1038/s41598-019-50974-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental procedures. (A) 2% agarose gels showing PCR products amplified from Nosema ceranae and Nosema apis DNA extracted from the spores captured on apiary tapes. M: Molecular weight marker (Gene ruler, 1-kb ladder, Thermo Scientific). Lanes 5–25 correspond to the experimental days represented by the tape fragments selected for the PCR analysis. (B) 2% agarose gels showing absence of PCR products amplified from Nosema ceranae and Nosema apis DNA extracted from the spores captured on tapes from the air in the Botanical Garden. M: Molecular weight marker (Gene ruler, 1-kb ladder, Thermo Scientific). Lanes 3–22 correspond to the experimental days represented by the tape fragments selected for the PCR analysis.