Fig. 3.

Encapsulation of LsLAI improves equilibrium conversion and provides thermal stability. a Loss of activity of encapsulated LsLAI (IC2) over time incubated in PBSM pH 7.4 at 37 °C (orange) or 50 °C (blue). Half-life calculated assuming first-order decay. b Equilibrium conversion of encapsulated LsLAI starting from 30 mM total substrate: 1:0 (30 mM galactose, purple), 1:1 (15 mM each, orange), or 0:1 (30 mM tagatose, green) galactose:tagatose, respectively. c Tagatose production from 200 mM galactose of L. plantarum wild type (WT) cells (green), IC2 cells expressing LsLAI intracellularly (I, purple), or crude lysate of the same IC2 cells expressing LsLAI cytoplasmically (LYS,orange) at different induction concentrations. The data are means from three biological replicates. Source data are provided as a Source Data file