Fig. 4.

Modified encapsulation of LsLAI maximizes tagatose production. Batch tagatose production over time starting from 300 mM galactose. Encapsulated LsLAI (IC2) was tested for tagatose production as unmodified (PBS) or SDS permeabilized (SDS) compared to purified enzyme at 37 °C or 50 °C to demonstrate its advantages. Encapsulated LsLAI is PBS-37C (green), encapsulated LsLAI at 50 °C is PBS-50C (pink), permeabilized encapsulated LsLAI at 50 °C is SDS-50C (blue), purified free-enzyme LsLAI at 37 °C is PURE-37C (orange), and purified free-enzyme LsLAI at 50 °C is PURE-50C (purple). Inset plot shows 0–1 h data to highlight initial reaction rates of each catalyst for comparison purposes. Salient features are summarized in table. The data are means from three biological replicates. Source data are provided as a Source Data file