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. 2019 Oct 7;9:14394. doi: 10.1038/s41598-019-50840-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of pan-HSPA inhibitors on cell viability and HSPAs expression in NSCLC cells. (A,C) Dose-response curves of VER-155008 (VER) (A) and JG-98 (C) in NSCLC cells. Cell viability was measured following 72 h treatment with VER (0–50 µM) or JG-98 (0–5 µM) using MTS assay. Results (mean ± SD from at least three independent measurements, each in three technical repeats) are expressed relatively to untreated control. (B,D) Expression of HSPA1, HSPA2, HSPA5, HSPA8 and HSPC proteins in NSCLC cell lines following 24 h treatment with VER or JG-98. Representative immunoblots are shown (n ≥ 3), actin was used as a protein loading control.