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. 2019 Oct 7;9:14394. doi: 10.1038/s41598-019-50840-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of NSCLC cells after single or combined treatment (72 h) with (A,B) VER-155008 (VER) and cisplatin (CDDP); (C) JG-98 and CDDP; (D–G) VER and bortezomib (BTZ); and (H,I) JG-98 and BTZ. Cell viability was measured using MTS assay. Results are expressed in relation to the untreated control (mean ± SD, n ≥ 3, each in triplicate). (G,J) Detection of dead cells following 72 h treatment of cells with VER (G), JG-98 (J), BTZ or both drugs. In (G) results of propidium iodide (PI) uptake test show mean values ± SD (n = 4, each in duplicate). In (J) results of lactate dehydrogenase (LDH) release assay are shown (mean values ± SD, n ≥ 4, each in duplicate). Statistical significance was determined using two-tailed t-test (A–C) or one-way analysis of variance (ANOVA) with Scheffe’s (D–G) or Duncan’s (H,I) adjustment for pairwise comparisons.